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Literature summary extracted from

  • Vincenzetti, S.; Cambi, A.; Neuhard, J.; Schnorr, K.; Grelloni, M.; Vita, A.
    Cloning, expression, and purification of cytidine deaminase from Arabidopsis thaliana (1999), Protein Expr. Purif., 15, 8-15.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.5 cDNA coding for AT-CDA1 amplified, subcloned into pTrc99-A, induced and expressed and overproduced in Escherichia coli SO5201 Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.5
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.5 3,4,5,6-Tetrahydrouridine
-
 Arabidopsis thaliana
3.5.4.5 3,4,5,6-Tetrahydrouridine
-
 Escherichia coli
3.5.4.5 3,4,5,6-Tetrahydrouridine
-
 Mus musculus
3.5.4.5 3-deazauridine
-
 Arabidopsis thaliana
3.5.4.5 5,6-Dihydrouridine
-
 Arabidopsis thaliana
3.5.4.5 5,6-Dihydrouridine
-
 Escherichia coli
3.5.4.5 5-fluoro-2'-deoxyuridine
-
 Arabidopsis thaliana
3.5.4.5 5-fluorouridine
-
 Arabidopsis thaliana
3.5.4.5 5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine
-
 Arabidopsis thaliana
3.5.4.5 CMP
-
 Arabidopsis thaliana
3.5.4.5 thymine riboside
-
 Arabidopsis thaliana
3.5.4.5 thymine riboside
-
 Escherichia coli
3.5.4.5 uridine
-
 Arabidopsis thaliana
3.5.4.5 uridine
-
 Escherichia coli
3.5.4.5 UTP
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.5 0.058
-
 cytosine arabinoside recombinant AT-CDA Arabidopsis thaliana
3.5.4.5 0.06
-
 deoxycytidine
-
 Escherichia coli
3.5.4.5 0.075
-
 deoxycytidine recombinant AT-CDA Arabidopsis thaliana
3.5.4.5 0.11
-
 cytidine
-
 Escherichia coli
3.5.4.5 0.15
-
 cytidine recombinant AT-CDA Arabidopsis thaliana
3.5.4.5 0.22
-
 cytosine arabinoside
-
 Escherichia coli
3.5.4.5 0.31
-
 5-azacytidine recombinant AT-CDA Arabidopsis thaliana
3.5.4.5 0.58
-
 5-methylcytidine recombinant Arabidopsis thaliana
3.5.4.5 0.95
-
 5-methylcytidine
-
 Escherichia coli
3.5.4.5 2.27
-
 5-azacytidine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.5 15000
-
 4 * 15000 Gallus gallus
3.5.4.5 32000
-
 2 * 32000, homodimer, SDS-PAGE Escherichia coli
3.5.4.5 32000
-
 2 * 32000, homodimer, SDS-PAGE Arabidopsis thaliana
3.5.4.5 32560
-
 calculated from cDNA open reading frame Arabidopsis thaliana
3.5.4.5 32580
-
 calculated from amino acid sequence Arabidopsis thaliana
3.5.4.5 63000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.5 cytidine + H2O Gallus gallus
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Haemophilus influenzae
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Bacillus subtilis
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Mus musculus
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Ovis aries
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Arabidopsis thaliana
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Escherichia coli SO5201
-
 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O Mus musculus BALB/c
-
 uridine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.5 Arabidopsis thaliana
-
-
-
3.5.4.5 Bacillus subtilis
-
-
-
3.5.4.5 Escherichia coli
-
-
-
3.5.4.5 Escherichia coli SO5201
-
-
-
3.5.4.5 Gallus gallus
-
 chicken
-
3.5.4.5 Haemophilus influenzae
-
-
-
3.5.4.5 Mus musculus
-
 mouse
-
3.5.4.5 Mus musculus
-
 BALB/c
-
3.5.4.5 Mus musculus BALB/c
-
 BALB/c
-
3.5.4.5 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.5
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.5 74
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.5 5-azacytidine + H2O
-
 Escherichia coli 5-azauridine + NH3
-
 ?
3.5.4.5 5-azacytidine + H2O
-
 Arabidopsis thaliana 5-azauridine + NH3
-
 ?
3.5.4.5 5-azacytidine + H2O
-
 Escherichia coli SO5201 5-azauridine + NH3
-
 ?
3.5.4.5 5-azadeoxycytidine + H2O
-
 Arabidopsis thaliana 5-azadeoxyuridine + NH3
-
 ?
3.5.4.5 5-methylcytidine + H2O
-
 Escherichia coli 5-methyluridine + NH3
-
 ?
3.5.4.5 5-methylcytidine + H2O
-
 Arabidopsis thaliana 5-methyluridine + NH3
-
 ?
3.5.4.5 5-methylcytidine + H2O
-
 Escherichia coli SO5201 5-methyluridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Gallus gallus uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Haemophilus influenzae uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Bacillus subtilis uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Mus musculus uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Ovis aries uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Arabidopsis thaliana uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Escherichia coli SO5201 uridine + NH3
-
 ?
3.5.4.5 cytidine + H2O
-
 Mus musculus BALB/c uridine + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Mus musculus uridine arabinoside + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Escherichia coli uridine arabinoside + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Arabidopsis thaliana uridine arabinoside + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Escherichia coli SO5201 uridine arabinoside + NH3
-
 ?
3.5.4.5 cytosine arabinoside + H2O
-
 Mus musculus BALB/c uridine arabinoside + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Mus musculus deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Escherichia coli deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Arabidopsis thaliana deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Escherichia coli SO5201 deoxyuridine + NH3
-
 ?
3.5.4.5 deoxycytidine + H2O
-
 Mus musculus BALB/c deoxyuridine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.5 dimer 2 * 32000, homodimer, SDS-PAGE Escherichia coli
3.5.4.5 dimer 2 * 32000, homodimer, SDS-PAGE Arabidopsis thaliana
3.5.4.5 tetramer
-
 Bacillus subtilis
3.5.4.5 tetramer 4 * 15000 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.5 3 10.5 deoxycytidine as substrate, specific activity constant in this range, beyond these values irreversible inactivation occurs Arabidopsis thaliana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.5 3 10.5 deoxycytidine as substrate, beyond these extremes rapid inactivation Arabidopsis thaliana